by oxygen blowing decarburization and electric silicon thermal method was studied, and the product quality of these two different processes was analyzed. The test data showed that the oxygen content of medium carbon ferromanganese alloy produced by electric silicon thermal method in China was between the oxygen content requirements of ordinary products and refined products abroad, and the fluctuation range was large (0.039-0.171%). According to the quality requirements of pipeline steel and its smelting process route, the change of total oxygen content of molten steel that may be caused by adding ordinary ferromanganese to adjust the manganese content of finished steel at the end of refining was estimated. In order to ensure the quality of clean steel production, it is recommended to use refined medium and low carbon ferromanganese alloy when adjusting the alloy element composition at the end of molten steel refining.
| C | P | S | ||||
| FeMn85C0.2 | 85 | 0.2 | 0.8-2.0 | 0.1-0.3 | 0.02 | |
| FeMn80C0.5 | 80 | 0.5 | 0.8-2.0 | 0.1-0.3 | 0.02 | |
| FeMn80C0.7 | 80 | 0.7 | 0.8-2.0 | 0.1-0.3 | 0.02 | |
1. protein isolated and purified from the nitrogen-fixing bacteria mutant UW3, which grows in nitrogen-fixing medium with Mn replacing molybdenum, can precipitate dark brown short oblique quadrangular prism crystals from the solution under certain crystallization conditions. The concentrations of Tris and Hepes buffer, NaCl, MgCl2 and PEG, and the crystallization method have a significant effect on the crystallization rate, number of crystal nuclei, crystal size and quality of the protein. The change of PEG concentration can also change the crystal form of the protein crystal. The optimal concentration of the above compounds required for the crystallization of MnFe protein is different from the optimal concentration required for the crystallization of the mutant ΔnifZMoFe protein lacking nifZ. SDS gel electrophoresis showed that the protein after the crystals were dissolved was basically the same as the MnFe protein before crystallization. The results showed that the crystals were crystals of MnFe protein.
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